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Hospital, Medical Center,Self High AccuracyAnti-TP ELISA Test Kit
INTENDED USE
The Anti-TP ELISA is a qualitative enzyme immunoassay for the in vitro detection of Treponema Pallidum (TP) infection, based on the detection of total antibody to Treponema Pallidum in human serum or plasma. It is intended for screening of blood donors and diagnosing patients related to infection with TP.
Product Detail
Description
Packaging Specifications
50 tests/box
Country Of Origin
China,BeiJing
Detection Limit
24 months
Storage
4-30℃
Specimen
serum, plasma, whole blood,fingertip blood
Instrument classification
Class I
Product Type
Repid Test
Accuracy
99.9%
Application
Hospital, Medical Center,self
ASSAY PROCEDURE
Prepare Reagents: Dilute 1 volume of Concentrated Washing Buffer (20×)with 19 volumes of distilled water, mix well.
Add Samples: Open the foil pouch and remove the Microplate. Set up 1 well as Blank, 2 wells as negative control, 2 wells as positive control. After dispensing 50μL of Sample Diluent , dispense 50μL of sample or negative control or positive control to the respective wells. Gently vibrating the plate.
Incubate: Cover the Microplate with plate cover and incubate the Coated Microplate in a thermostat-controlled water-bath or microplate incubator at 37℃ for 60 minutes.
Wash the Plate: Remove the plate cover. Aspirate the contents of all wells. Fill the wells with the diluted washing buffer ( 10~20 seconds to soak) then aspirate again. Repeat the procedure for 5 times. Make sure that the rest volume is minimal, by tapping plate onto absorbent paper.
Add Conjugate: Add 100μL of conjugate to each well (except for the blank well).
Incubate: Cover the Microplate and incubate the plate at 37℃ for 30 minutes.
Wash the Plate: Repeat the wash procedure as in step 4.
Add Substrate: Add 50μL of Substrate Solution A and 50μL of Substrate Solution B to each well, mix well. Cover and incubate at 37℃ for 30 minutes.
Stop reaction: Add 50μL Stop Solution to each well, mix well.
Read the absorbance at 450 nm. If a dual wavelength measurement is used, the reference wavelength should be selected from 620nm to 690nm.
PRECAUTIONS
Allow all kit components to reach room temperature before use.
Follow the direction insert to control the reaction temperature and time strictly.
Do not mix components of different lot numbers to use.
The kit should be store at 2-8℃. Do not use kit components beyond their expiration date.
Biovantion Inc is independent from international department of Bioneovan Co., Ltd., which established in 2 0 0 5, is an in vitro diagnostic reagents manufacturer engaged in the research, development, production, and focus on clinical testing for infectious diseases, such as Hepatitis virus, Noro vir... Biovantion Inc is independent from international department of Bioneovan Co., Ltd., which established in 2 0 0 5, is an in vitro diagnostic reagents manufacturer engaged in the research, development, production, and focus on clinical testing for infectious diseases, such as Hepatitis virus, Noro vir...