Biovantion Inc.
| Payment Terms | L/C, Western Union, T/T |
| Supply Ability | 1000kits/week |
| Delivery Time | 5-8 working days |
| Packaging Details | 96wells/box or 48wells/box |
| Short names | IGF-I |
| Cat. No. | In-Hu3783 |
| Gene names | IBP1 |
| Standard | 90ng/ml |
| Work | Serum, plasma, culture media or any biological fluid |
| Alternative names | Mechano growth factor |
| Model Number | In-Hu3783 |
| Place of Origin | CHINA |
| Brand Name | BIOVANTION |
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Product Specification
| Payment Terms | L/C, Western Union, T/T | Supply Ability | 1000kits/week |
| Delivery Time | 5-8 working days | Packaging Details | 96wells/box or 48wells/box |
| Short names | IGF-I | Cat. No. | In-Hu3783 |
| Gene names | IBP1 | Standard | 90ng/ml |
| Work | Serum, plasma, culture media or any biological fluid | Alternative names | Mechano growth factor |
| Model Number | In-Hu3783 | Place of Origin | CHINA |
| Brand Name | BIOVANTION | ||
| High Light | Mechano Growth Factor MGF ELISA Kit ，MGF ELISA Kit ，Insulin Like Growth Factor 1 Test | ||
Human Insulin-like growth factor 1;IGF-1;Mechano growth factor;MGF ELISA Kit
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to IGF-1. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for IGF-1 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain IGF-1 and HRP conjugated IGF-1 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of IGF-1. You can calculate the concentration of IGF-1 in the samples by comparing the OD of the samples to the standard curve.
Materials provided with the kit
| Materials provided with the kit | 48 determinations | 96 determinations | Storage | |
| 1 | User manual | 1 | 1 | R.T. |
| 2 | Closure plate membrane | 2 | 2 | R.T. |
| 3 | Sealed bags | 1 | 1 | R.T. |
| 4 | Microelisa stripplate | 1 | 1 | 2-8℃ |
| 5 | Standard:90ng/ml | 0.5ml×1 bottle | 0.5ml×1 bottle | 2-8℃ |
| 6 | Standard diluent | 1.5ml×1 bottle | 1.5ml×1 bottle | 2-8℃ |
| 7 | HRP-Conjugate reagent | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
| 8 | Sample diluent | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
| 9 | Chromogen Solution A | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
| 10 | Chromogen Solution B | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
| 11 | Stop Solution | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
| 12 | Wash solution | 20ml (20X)×1bottle | 20ml (30X)×1bottle | 2-8℃ |
Calculation of Results
Known concentrations of Human IGF-1 Standard and its corresponding reading OD is plotted on the log scale (x-axis) and the log scale (y-axis) respectively. The concentration of Human IGF-1 in sample is determined by plotting the sample’s O.D. on the Y-axis. The original concentration is calculated by multiplying the dilution factor.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Human IGF-1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Human IGF-1were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Assay range
1.6 ng/ml -70 ng/ml
Sensitivity:
0.5 ng/ml
Company Details
Business Type:
Manufacturer,Importer,Exporter,Trading Company,Seller
Ecer Certification：
Verified Supplier
Biovantion Inc is independent from international department of Bioneovan Co., Ltd., which established in 2 0 0 5, is an in vitro diagnostic reagents manufacturer engaged in the research, development, production, and focus on clinical testing for infectious diseases, such as Hepatitis virus, Noro vir... Biovantion Inc is independent from international department of Bioneovan Co., Ltd., which established in 2 0 0 5, is an in vitro diagnostic reagents manufacturer engaged in the research, development, production, and focus on clinical testing for infectious diseases, such as Hepatitis virus, Noro vir...
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