HIV Ab Ag Elisa Test Kits With 18-Month Shelf Life And High Sensitivity

Product Description:

This is a 4th-generation assay for the simultaneous detection of antibodies to HIV-1 and HIV 2 as well as p24 antigen of HIV-1. The assay is based on recombinant and synthetic peptides for antibody detection and monoclonal antibody specific to the p24 protein of HIV-1. It has great significance in the diagnosis of HIV infection / screen for donated blood to prevent transmission of HIV to recipients and as an aid in clinical diagnosis of HIV-related infections.

Applications:

1. Prepare Reagents: Dilute 1 volume of Washing Buffer with 19 volumes of distilled water, mix well.

2. Add Conjugate 1 and Samples: Open the foil pouch and remove the Microplate. Set up 1 well as Blank, 2 wells as Negative Control, 2 wells as Positive Control HIV-1, 2 wells Positive Control HIV-2 and 2 wells Positive Control P24 Antigen. After dispensing 75μL of sample or Negative Control or Positive Control to the respective wells, dispense 25μL of Conjugate 1 to each well (except the blank well). Gently vibrating the plate.

3. Incubate: Cover the Microplate with plate cover and incubate the Microplate in a thermostat-controlled water-bath or microplate incubator at 37℃ for 60 minutes.

4. Wash the Plate: Remove the plate cover. Aspirate the contents of all wells. Fill the wells with the diluted washing buffer (10~20 seconds to soak) then aspirate again. Repeat the procedure for 5 times. Make sure that the rest volume is minimal, by tapping plate onto absorbent paper.

5. Add Conjugate 2: Add 100μL of Conjugate 2 to each well (except the blank well).

6. Incubate: Cover the Microplate and incubate the plate at 37℃ for 30 minutes.

7. Wash the Plate: Repeat the wash procedure as in step 4.

8. Add Substrate: Add 50μL of Substrate Solution A and 50μL of Substrate Solution B to each well, mix well. Cover and incubate at 37℃ for 30 minutes.

9. Stop reaction: Add 50μL Stop Solution to each well, mix well.

10. Read the absorbance at 450 nm. If a dual wavelength measurement is used, the reference wavelength should be selected from 620nm to 690nm.

Precautions:

1. Allow all kit components to reach room temperature before use.

2. Follow the direction insert to control the reaction temperature and time strictly.

3. Do not mix components of different lot numbers to use.

4. The kit should be store at 2-8 . Do not use kit components beyond their ℃ expiration date.

FAQ:

Q: What is the brand name of the Elisa Test Kit?

A: The brand name of the Elisa Test Kit is Biovantion.

Q: What is the model number of the Elisa Test Kit?

A: The model number of the Elisa Test Kit is BVTE0097.

Q: Where is the Elisa Test Kit manufactured?

A: The Elisa Test Kit is manufactured in China.

Q: What certification does the Elisa Test Kit have?

A: The Elisa Test Kit has ISO13485 certification.

Q: What is the minimum order quantity for the Elisa Test Kit?

A: The minimum order quantity for the Elisa Test Kit is 10.

Q: What is the price of the Elisa Test Kit?

A: The price of the Elisa Test Kit is negotiable.

Q: What are the packaging details for the Elisa Test Kit?

A: The packaging details for the Elisa Test Kit are carton.

Q: What is the delivery time for the Elisa Test Kit?

A: The delivery time for the Elisa Test Kit is 7-15 days.

Q: What are the payment terms for the Elisa Test Kit?

A: The payment terms for the Elisa Test Kit are TT 100% payment.

Q: What is the supply ability of the Elisa Test Kit?

A: The supply ability of the Elisa Test Kit is 100000.