25-OH Vitamin D Total

Product Description:

Vitamin D is a fat-soluble ster0id hormone precursor that is mainly produced in the skin by exposure to sunlight. Vitamin D is biologically inert and must undergo hydroxylation steps to become active.1 Our body can only synthesize vitamin D3. Vitamin D2 is taken up with fortified food or given by supplements. Physiologically, vitamin D3 and D2 are bound to the vitamin D-binding protein (VDBP) in plasma and transported to the liver to become 25- hydroxyvitamin D (25-OH Vitamin D). As vitamin D (25-OH) represents the major storage form, its blood concentration is used to assess the overall vitamin D status. More than 95% of vitamin D (25-OH), measurable in serum, is vitamin D3 (25-OH) whereas vitamin D2 (25- OH) reaches measurable levels only in patients taking vitamin D2 supplements.1,2,3 Vitamin D is essential for bone health. In children, severe deficiency leads to rickets. In elderly, the risk of falling has been attributed to vitamin D deficiency due to muscle weakness. Moreover, low vitamin D (25-OH) concentrations are associated with lower bone mineral density. Insufficiency has also been linked to diabetes, cardiovascular disease, and autoimmune diseases.

Features:

• Product Name: 25-Hydroxyvitamin D Total (ELISA)
• Manufacturer: Biovantion
• Country of Origin: China
• Specificity: High
• Kit Size: 96 Tests
• Shelf Life: 18 Months

The Elisa Test Kit is an Antibody Detection Assay Kit for ELISA Testing designed and manufactured by Biovantion in China. It is a highly specific ELISA Diagnostic Test Kit for Infectious Diseases, with a kit size of 96 tests and a shelf life of 18 months. The kit utilizes an Antigen-Antibody Binding Test Kit for ELISA, providing accurate and reliable results for diagnostic purposes.

Applications:

The Vitamin D total assay is an enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of total 25-hydroxy Vitamin D (Vitamin D total) in human serum. Results are to be used in conjunction with other clinical and laboratory data to assist the clinician in the assessment of vitamin D sufficiency. For professional use only.

Test principle:

Competitive principle. Total duration of assay: 85 minutes. • Sample, 25-Hydroxyvitamin D derivant coated microwells and enzyme labeled Anti- 25- Hydroxyvitamin D are combined. • During the incubation, 25-Hydroxyvitamin D derivant coated on microwells and 25- Hydroxyvitamin D present in the sample compete for binding to the enzyme labeled antibodies. • After washing, a complex is generated between the solid phase and enzymelinked antibodies by immunological reactions. • Substrate solution is then added and catalyzed by this complex, resulting in a chromogenic reaction. The resulting chromogenic reaction is measured as absorbance. • The color intensity is inversely proportional to the amount of 25-Hydroxyvitamin D in the sample.