Product Specification

Diagnostic Kit for Human Parvovirus B19 IgM Antibody (ELISA)

APPLICATIONS:

This kit is used for qualitative detection of parvovirus B19 IgM antibody in serum or plasma from pregnant women and pregnant women. Human parvovirus B19 is transmitted through the respiratory tract, pregnant women susceptible to up to 23%~45%, if pregnancy women infected with B19, the fetus infection rate is 33% through the placenta, the incidence of stillbirth 6.5%, the incidence of non-immune fetal edema 8%~10%. Specific IgM and IgG antibodies are tested to diagnose whether pregnant women are infected with B19 virus. The appearance of IgG antibodies indicates previous infection, IgG antibodies remain positive all their lives and generally obtain lifelong immunity. If pregnant women have a certain level of anti-b19 virus specific IgG, it can protect the fetus, reduce the damage of B19 virus infection. Serological test to detect specific IgG and IgM antibodies is the basis for the diagnosis of B19 infection. Antibody detection methods mainly include radioimmunoassay (RIA) enzyme immunoassay (ELISA) immunofluorescence assay (IF) and immunoblot assay (WB). This kit assists in the diagnosis of past infection of parvovirus B19 in pregnant women.

COMPONENTS:

Materials provided with the kits:

Materials required but not provided:

• Micropipettes: 0.02, 0.05, 0.10, 0.15, 0.20, and 1.0 ml.
• Disposable pipette tips.
• Distilled or deionized water.
• Humidified Box capable of maintaining 37°C
• Absorbent paper or paper towel.
• Microtiter plate or strip-well washer
• Microtiter plate reader with 450nm (or 450 nm/630 nm) wavelength
• Timer

 
PRECAUTION FOR USERS:

• For in-vitro diagnostic use only.
• Do not use kit beyond expiration date.
• Do not mix components from kits with different lot number.
• Avoid microbial contamination of reagents.
• Do not pipet reagent by mouth and do smoking or eating while performing assays.

• Wear gloves during the whole process and avoid reagents or specimen spilling-out.
• Wipe up the spills using 5% hypochlorite solution.
• Decontaminate all liquids or solid wastes before deposing.

 
 
SUPPORT CUSTOMIZATION: 
 

Biovantion's Elisa test kits can be customized to your needs, making them the perfect solution for your laboratory's needs. Our kits provide highly specific, colorimetric detection methods in a convenient 96-kit. With add-ons like detection buffers and antibody-coated plates, you can be sure of excellent results every time.

Contact BIOVANTION to get the Elisa test kit that satisfies you!
 
PACKAGING:

Elisa test kits are carefully packaged and shipped in an insulated box to ensure the temperature is maintained during transport. The package contains the test kit, instructions, and other supplies needed for the test.

It also includes a return label for customers to return unused portions of the kit after testing is complete. All packages come with tracking information, so customers can track the progress of their shipment.
 
STORAGE OF THE TEST KIT:

Unopened test kits should be stored at 2-8C upon receipt and the microtiter plate should be kept in a sealed bag to minimize exposure to damp air. Use up the reagents as soon as possible after the kit is unpacked.
 
ASSAY PROCEDURE: 

• Bring ELISA Kit (all reagents), and samples to room temperature before use (approximately 30 minutes).
• Dilute concentrated wash buffer 1:19 with ddH₂O.
• For each test, set one blank, two positive and three negative controls. Add 100μl positive and negative control serum into positive and negative control wells respectively.
• Add 100μl sample diluent into test wells, then add 10μl specimen in each well.
• Cover wells with seal paper, then incubate 30 minutes at 37°C.
• Wash each well 5 times by filling each well with diluted wash buffer, then inverting the plate vigorously to get all water out and blocking the rim of wells on absorbent paper for 15 seconds.
• Add 100 μl Enzyme Conjugant to each well except the blank well.
• Incubate: Cover the Microplate with plate cover and incubate the Coated Microplate in a thermostat-controlled water-bath or microplate incubator at 37℃ for 30 minutes.
• Wash the Plate as step 6.
• Add Substrate: Add 50μL of Substrate Solution A and 50μL of Substrate Solution B to each well, include blank. mix well. Cover and incubate at 37℃ for 15 minutes.
• Stop reaction: Add 50μL Stop Solution to each well, include blank mix well.
• Read the absorbance at 450 nm. If a dual wavelength measurement is used, the reference wavelength should be selected from 630nm to 690nm.

INTERPRETATION OF RESULTS:

• Colorimetric Method
• Cut off Value calculation:
• COV = 0.10 + the average OD of negative controls (If the absorbance of negative controls is below 0.05, calculate it as 0.05.If the absorbance of negative controls is above 0.05; calculate it as its original value.)
• Positive OD450 of sample ≥ COV HPVB19- IgM
• Negative OD450 of sample ＜ COV. HPVB19- IgM