PCR detect method Monkeypox detect reagent mpox virus PCR Reagent with 48 test

Product Description:

Monkeypox(MPX) is an acute zoonotic infectious disease caused by Monkeypox Virus(MPXV). Monkeypox virus usually invades the body through the respiratory mucosa, propagates in the lymphocytes and invades the blood, produces temporary viremia, multiplies in the cells, and then invades the blood from the cells, runs to the skin of the whole body, and causes lesions. The clinical symptoms of monkeypox infection are similar to those of Variola, but are usually milder. Generally, after a 12-day incubation period, fever, headache, muscle pain, and swollen lymph nodes appear. Papules appear 1-3 days after fever, and papules will appear on the face or other parts of the body. As the disease progresses, the pimples crust and fall off. The course of the disease generally lasts 2-4 weeks, and the mortality rate is 1%-10%. Lymphadenopathy is one of the main differences between this disease and smallpox.

In recent years, the diagnostic technology of monkeypox virus has been greatly developed, from the earliest virus isolation and serological methods to the current ELISA and polymerase chain reaction (PCR). Nucleic acid testing is the gold standard for diagnosing monkeypox infection.

Features:

• Product Name: PCR Reagent
• Reaction Conditions: Standard
• Positive Control: Plasmid DNA
• Method: PCR
• Storage Condition: 2-35℃
• Quality Control: Endonuclease And Exonuclease Activity Tested

Polymerase Chain Reaction Reagent, DNA amplification reagent, Polymerase chain reaction reagent kit

PRECAUTIONS

1. The sample preservation solution contains virus lysis components. After the sampling swab is added to the sampling solution, the virus in the sample can be completely inactivated in about 5 minutes and can be operated safely.

2. When squeezing the body of the reagent bottle, it is normal to remove any liquid. The liquid is non-toxic and harmless. If it splashes on the skin, wipe it with clean water.

3. In the process of absorbing the mixed enzyme into the reaction solution bottle, the residual enzyme in the mixed enzyme tube can be washed into the reagent bottle with the liquid in the reaction solution bottle.

4. It is recommended to use the commercial nucleic acid purification kit to extract nucleic acid for the inter room quality control products added with preservatives and the collection tubes with interference, and then use this kit for detection.

5. If two drops of reagent and sample are accidentally added during dropping, the test results will not be affected.

Technical Parameters:

MATERIALS AND EQUIPMENT REQUIRED BUT NOT SUPPLIED

1. The compatible PCR instruments: YS-qPCR-1 or SLAN-96P or ABI 7500 RT-PCR system.

2. Mini centrifuge with rotor for centrifuge tubes.

3. Mini centrifuge with rotor for PCR tubes.

4. Nuclease-free centrifuge tubes.

5. Nuclease-free PCR tubes and caps.

6. Adjustable pipettors and filtered pipette tips for handling DNA.

7. Tube racks.

8. Disposable powder-free gloves.

STORAGE AND EXPIRATION DATE:

1. Store the kits at 2-35℃. MPXV Reagent Strip (Freeze-Dried) and Positive Control (Freeze-Dried) can be transported at room temperature.

2.The dissolved Positive Control needs to be stored at -20±5℃ and it could be stored for 1 year.

3.The shelf-life of the kit is 12 months. After disassembling, it is recommended to store tightly.

4.Always check the expiration date before use, and do not use any expired reagent. For the manufacturing date and expiration date, see the outer packaging box.

5.After opening, the Kit is stable up to the expiration date indicated on the packaging provided that the components have been stored correctly according to the recommendations.