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{"title":"96 Test Aflatoxin M1 Mycotoxin ELISA Kit For Milk","imgUrl":"https:\/\/img.chinax.com\/nimg\/12\/7f\/571b8df5bc628dbe26a9a59b6dc2-200x200-1\/96_test_aflatoxin_m1_mycotoxin_elisa_kit_for_milk.jpg","attrs":{"Brand Name":"REAGEN","Model Number":"RNM98001","Certification":"ISO","Place of Origin":"USA"}}
{"title":"High Sensitivity AflaPure M1 Kit For Downstream HPLC And ELISA Analysis","imgUrl":"https:\/\/img.chinax.com\/nimg\/9e\/5c\/30ffb5aec52c755d12c5be14f857-200x200-1\/high_sensitivity_aflapure_m1_kit_for_downstream_hplc_and_elisa_analysis.jpg","attrs":{"Brand Name":"REAGEN","Model Number":"RNM98010","Certification":"","Place of Origin":"USA"}}
{"title":"AflaPure Total Aflatoxin Rapid Immunoaffinity Columns Kit Color Packing","imgUrl":"https:\/\/img.chinax.com\/nimg\/d4\/e6\/7eabc7e274e314c967c91821e250-200x200-1\/aflapure_total_aflatoxin_rapid_immunoaffinity_columns_kit_color_packing.jpg","attrs":{"Brand Name":"REAGEN","Model Number":"RNM98011","Certification":"","Place of Origin":"USA"}}
{"title":"Fumonisin Toxin Mycotoxin ELISA Kit Stored 2-8 Degrees High Reproducibility","imgUrl":"https:\/\/img.chinax.com\/nimg\/76\/92\/e6afc900394107bbdba17a1b009b-200x200-1\/fumonisin_toxin_mycotoxin_elisa_kit_stored_2_8_degrees_high_reproducibility.jpg","attrs":{"Brand Name":"REAGEN","Model Number":"RNM98009","Certification":"","Place of Origin":"USA"}}
{"title":"Mycotoxin ELISA Kit Ochratoxin A ELISA Test Kit For Cereals Meat Feed Milk Urine","imgUrl":"https:\/\/img.chinax.com\/nimg\/09\/46\/9f900f287fdd25f2b0dccb67d7a1-200x200-1\/mycotoxin_elisa_kit_ochratoxin_a_elisa_test_kit_for_cereals_meat_feed_milk_urine.jpg","attrs":{"Brand Name":"REAGEN","Model Number":"RNM98008","Certification":"","Place of Origin":"USA"}}
Label the individual strips that will be used and aliquot reagents as the following example:
Component
Volume per Reaction
24 Reactions
Kanamycin Antibody #1
50uL
1.2mL
HRP-Conjugated Ab #2
50uL
1.2mL
1X Wash Solution
1.0 mL
24mL
Stop Buffer
100 uL
2.4 mL
TMB Substrate
100 uL
2.4 mL
Add 50 uL of each Kanamycin Standards in duplicate into different wells (Add standards to plate only in the order from low concentration to high concentration).
Add 50 uL of each sample in duplicate into different sample wells.
Add 50 uL of HRP-Conjugated Ab#2 to each well and 50 uL of Kanamycin Antibody #1 to each well. Mix well by gently rocking the plate manually for 30s.
Incubate the plate for 30 minutes at room temperature (20 – 25°C / 68 – 77°F). (Avoid direct sunlight and cold bench tops during the incubation. Covering the microtiter plate while incubating is recommended).
Wash the plate 4 times with 250 uL of 1X Wash Solution. After the last wash, invert the plate and gently tap the plate dry on paper towels (Perform the next step immediately after plate washings. Do not allow the plate to air dry between working steps).
Add 100 uL of TMB substrate. Time the reaction immediately after adding the substrate. Mix the solution by gently rocking the plate manually for 30s while incubating(Do not put any substrate back to the original container to avoid any potential contamination. Any substrate solution exhibiting coloration is indicative of deterioration and should be discarded. Covering the microtiter plate while incubating is recommended).
After incubating for 15 minutes at room temperature (20 – 25°C / 68 – 77°F), add 100 uL of Stop Buffer to stop the enzyme reaction.
Read the plate as soon as possible following the addition of Stop Buffer on a plate reader with 450 nm wavelength (Before reading, use a lint-free wipe on the bottom of the plate to ensure no moisture or fingerprints interfere with the readings).
Company Details
Bronze Gleitlager
,
Bronze Sleeve Bushings
and
Graphite Plugged Bushings
from Quality China Factory
Business Type:
Manufacturer
Year Established:
2012
Total Annual:
15000000-20000000
Employee Number:
50~80
Ecer Certification：
Verified Supplier
REAGEN INC
For more than 13 years REAGEN has been focusing on best-in-class PCR, NGS, ELISA, and lateral flow rapid test kits for human and animal infectious disease diagnostics, as well as for food safety testing.
REAGEN develops and manufactures diagnostic kit s for the detection of infectious...
REAGEN INC
For more than 13 years REAGEN has been focusing on best-in-class PCR, NGS, ELISA, and lateral flow rapid test kits for human and animal infectious disease diagnostics, as well as for food safety testing.
REAGEN develops and manufactures diagnostic kit s for the detection of infectious...
