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Amp-Future (Changzhou) Biotech Co., Ltd.

  • China,Changzhou ,Jiangsu
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China High Sensitivity RNA Amplification With Improved Detection Isothermal Kit
China High Sensitivity RNA Amplification With Improved Detection Isothermal Kit

  1. China High Sensitivity RNA Amplification With Improved Detection Isothermal Kit

High Sensitivity RNA Amplification With Improved Detection Isothermal Kit

  1. MOQ: 16T
  2. Price: USD3.8$/T
  3. Get Latest Price
Payment Terms T/T,Paypal
Supply Ability 100000T/Month
Delivery Time 6days
Packaging Details 16T/bag,48T/box
Product Name DNA Isothermal Amplification Kit EXO
Shelf Life 14 Months
Reaction Volume 50 μL
Application Nucleic Acid Amplification
Storage Temperature -20℃
Kit Type Amplification
Components Primers, Polymerase, Buffer, DNTPs
Reaction Time 20mins
Brand Name Amp-future bio
Model Number WLE8202KIT
Certification ISO9001
Place of Origin China

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  1. Product Details
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Product Specification

Payment Terms T/T,Paypal Supply Ability 100000T/Month
Delivery Time 6days Packaging Details 16T/bag,48T/box
Product Name DNA Isothermal Amplification Kit EXO Shelf Life 14 Months
Reaction Volume 50 μL Application Nucleic Acid Amplification
Storage Temperature -20℃ Kit Type Amplification
Components Primers, Polymerase, Buffer, DNTPs Reaction Time 20mins
Brand Name Amp-future bio Model Number WLE8202KIT
Certification ISO9001 Place of Origin China
High Light High Sensitivity rna amplification20mins rna amplificationIsothermal rna amplification methods

High Sensitivity RNA Amplification With Improved Detection Isothermal Kit

Product Detail

Applicable equipment:

It is recommended to use the Isothermal fluorescence detector developed by Amp-future, which is also suitable for fluorescence quantitative PCR apparatus with market known brands.

 

Kit Storage and term of Validity

Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.

Term of Validity: 14 months

 

Isothermal nucleic acid Principle Summary

This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39℃~42℃), with the help of auxiliary proteins and single-strand binding proteins,the recombinase and primers form a complex;Source search and combine the target homology domain, at this time,a D-loop region is formed at the homology position and strand exchange begins;along with the dissociation of the recombinase from the complex,the polymerase also binds to the 3' end of the primer and begins chain extension. At the same time, relying on the function of exonuclease, adding specific molecular probes designed according to the template, and using fluorescence monitoring equipment can realize real-time monitoring of the amplification process of the target fragment.

Isothermal nucleic acid Product Features

1/ High sensitivity and specificity, short reaction time.

2/ The reagent form is freeze-dried, stable and easy to operate.

 

Technical Parameters:
Parameters Details
Product Name DNA Isothermal Amplification Kit EXO
Manufacturer Amp-future
Storage Temperature -20°C
Kit Components Enzymes, Buffers ,Reagents
Packaging 48 Tests/box
Detection Limit 500-1000copies/µL
Shipping ICE
Test Time 5-20mins

 

Isothermal nucleic acid Applications

Suitable for DNA isothermal rapid amplification kit(fluorescent type)

Primer: Require pair of nucleotide primers with the length of 25-35 bp.

Fluorescent Probe:Require the suitable length is 46-52nt.

DNA fluorescent kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.

 

Notes

1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.

2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.

Company Details

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 from Quality China Factory
  • Business Type:

    Manufacturer,Importer,Exporter

  • Year Established:

    2021

  • Total Annual:

    100000-500000

  • Employee Number:

    50~80

  • Ecer Certification:

    Active Member

MIRA (Multienzyme Isothermal Rapid Amplification) - a multienzyme isothermal rapid amplification technology based on genetic recombination and repair mechanism in organisms, relying on the synergy of multiple functional proteins at room temperature (39°C-42°C) to achieve rapid nucleic acid a... MIRA (Multienzyme Isothermal Rapid Amplification) - a multienzyme isothermal rapid amplification technology based on genetic recombination and repair mechanism in organisms, relying on the synergy of multiple functional proteins at room temperature (39°C-42°C) to achieve rapid nucleic acid a...

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  • Amp-Future (Changzhou) Biotech Co., Ltd.
  • Floor 4, Building 9#, Innovation Park, Changzhou, Israel, China, No. 18-67, Changwu Middle Road, Wujin District, Changzhou City, Jiangsu Province
  • https://www.ampfuturebio.com/

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