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Shenzhen Lvshiyuan Biotechnology Co.,Ltd

  • China,Shenzhen ,Guangdong
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China LSY-20052 Nitrofuran(AHD) rapid test strips (Honey) honey rapid test
China LSY-20052 Nitrofuran(AHD) rapid test strips (Honey) honey rapid test

  1. China LSY-20052 Nitrofuran(AHD) rapid test strips (Honey) honey rapid test

LSY-20052 Nitrofuran(AHD) rapid test strips (Honey) honey rapid test

  1. MOQ: 1 kit
  2. Price: FOBShenzhen$192/kit
  3. Get Latest Price
Payment Terms T/T, Western Union, MoneyGram
Delivery Time 3 days
Packaging Details By foam box with ice to maintain cool storage 16.5*12.5*12.5cm
Specifications 96 tests/kit
Sample performance Honey, tissue, egg
Detection limit 0.5ppb
Shelf life 12 months
Brand Name Green Spring
Model Number LSY-20052
Certification ISO9001:2008
Place of Origin China

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  1. Product Details
  2. Company Details

Product Specification

Payment Terms T/T, Western Union, MoneyGram Delivery Time 3 days
Packaging Details By foam box with ice to maintain cool storage 16.5*12.5*12.5cm Specifications 96 tests/kit
Sample performance Honey, tissue, egg Detection limit 0.5ppb
Shelf life 12 months Brand Name Green Spring
Model Number LSY-20052 Certification ISO9001:2008
Place of Origin China

Nitrofuran(AHD) rapid test dipsticks (Honey)

Catalog No. LSY-20052

  • Specifications

96 strips/kit

2. Brief

This product is used for testing Nitrofuran(AHD) in honey sample qualitatively. Easy to operate, high sensitivity.

3. Detection limit

Honey: 0.5ng/g (0.5ppb)

4. Contents

1 Nitrofuran(AHD) test dipsticks bottle(include test dipsticks, desiccant, micro-wells reagent) 12 bottles
2 Manual 1 piece
3 1M HCl 2 bottles
4 1M NaOH 2 bottles
5 1M K2HPO4 solution 2 bottles
6 Derivatization reagent 3 bottles
7 Sample buffer 2 bottles

5. Principle

The Nitrofuran(AHD) rapid test dipstick is based on competitive inhibition immuno-chromatographic principle. In the flow process, Nitrofuran(AHD) in the sample combined with Nitrofuran(AHD) specific colloidal gold-labeled monoclonal antibody, Inhibit the combination between antibody and Nitrofuran(AHD)-BSA conjugate on Test line of NC membrane, lead to the color change of Test line. When the sample has no Nitrofuran(AHD) residue or concentration lower than detection limit, T line is darker than C line or T line has same color with C line; when the concentration is equal to or higher than detection limit, T line is obviously lighter than C line or T line has no color. No matter whether there is Nitrofuran(AHD) residue in sample, C line will appear, it means the test is valid.

6. Sample preparation

1) 0.1M K2HPO4 solution: Dilute 1M K2HPO4 solution with deionized water at 1:9.

2) Honey: Take 3±0.05g honey sample into 50ml centrifuge tube, add 6ml deionized water,750ul 1M HCl and 300ul Derivatization reagent, shake and mix for 2min, put at 60℃ water bath for 30min;

3) Add 7.5ml 0.1M K2HPO4 solution,600ul 1M NaOH and 10ml ethyl acetate, shake and mix for 1min, centrifuge at 4000r/min for 10min;

4) Take 5ml up-layer liquid into glass tube, blow to dry at 56℃ by Nitrogen or air.

5) Add 2ml n-hexane shake to dissolve the dry residue (if do not dissolve the residue completely, it may lead to the result unstable), then add 0.6ml sample buffer, shake to even for 30s, be stable until layered, absorb out the up-layer(if appear emulsion, centrifuge at 4000r/min for 5min);

6) Absorb 200ul down-layer liquid (water layer), ready to test. (Avoid absorb the up-layer liquid).

7. Operation procedures

7.1 Read the instruction carefully before use. Return test dipsticks and sample into room temperature.

7.2 Take bottles needed from the kit package, take out required microwells and dipsticks, making proper marks. Please use these test dipsticks within 1h. Seal the cap of the bottles, avoid moisture.

7.3 Take 200ul of the test samples into the microwells, then repeatedly absorb for 5-10 times, mix the sample with the reagent in the microwells completely until no solid judged by eyes (this is a very important step).

7.4 Incubate for 5min at room temperature (20-25℃), insert the test dipsticks into the microwells with the "MAX" end fully dipped in to the mixture solution.

7.5 Incubate for 5-8min at room temperature again, read the result, it is invalid in other time.

8. Test Result Interpretation

8.1 Negative: T line is darker than C line, or T line has same color with C line. It means there is no Nitrofuran(AHD) residue in sample or the residue is lower than detection limit.

8.2 Positive: T line is obviously lighter than C line or T line is invisible. It means the Nitrofuran(AHD) residue is equal to or higher than detection limit.

8.3 Invalidation: C line isn’t seen wine red. It means the test card is out of efficacy, out of date or improper operation. Please run the test again using another package. If the invalid tests keep happening, please contact the supplier.

 

 

 

 

9. Precautions

1) The test card can be used only once at room temperature, do not use test card out of expiry date.

2) Do not repeat use the tips to avoid cross pollution.

3) Do not touch the white membrane surface in the middle of test card, avoid sunlight and fan blowing directly.

4) Wear gloves when testing.

5) Please contact the supplier for any questions.

10.Specificity

There is no cross reaction with Nitrofuran AMOZ, AOZ and SEM.

11. Storage and expiry date

Storage: Store at 2-8 ℃ in dark, sealed, dry place, no frozen.

Expiry date: 12 months; date of production is on box.

 

 

Company Details

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,

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 and 

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 from Quality China Factory
  • Business Type:

    Manufacturer,Exporter

  • Year Established:

    2003

  • Total Annual:

    $100,000,000-$200,000,000

  • Employee Number:

    200~500

  • Ecer Certification:

    Site Member

Shenzhen Lvshiyuan Biotechnology Co.,Ltd(hereinafter referred to as LSY), was founded in 2003, located in the National Marine Biological Industrial Park (Dapeng New District, Shenzhen), with an area of more than 20000 square meters according to GMP standards. We have established five branches ... Shenzhen Lvshiyuan Biotechnology Co.,Ltd(hereinafter referred to as LSY), was founded in 2003, located in the National Marine Biological Industrial Park (Dapeng New District, Shenzhen), with an area of more than 20000 square meters according to GMP standards. We have established five branches ...

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Get in touch with us

  • Reach Us
  • Shenzhen Lvshiyuan Biotechnology Co.,Ltd
  • D building, National Biological Industrial Park of Marinelife, No.2 Binghai Road, Dapeng, Shenzhen, China 518120
  • MrsBella Zou
  • +86-18165709090
  • https://www.lsybtbio.com/

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